Instrumentation

The following equipment and instruments are used for experiments on a daily basis:

X-Ray Data Collection

In-house Cu rotating anode X-ray source equipped with a rigaku CCD Saturn 944+ and an R-AXIS IV imaging plate detector.

 

Crystallization

Crystallization room (controlled temperature and humidity) and crystallization robot (Thermo Hydra II).

 

Protein Purification

Several GE Healthcare AKTA protein purification systems (2 FPLCs, 2 Prime Plus, and 1 Pure)

 

Wet Lab

Incubators for cell cultures, UV plate reader, fluorescence spectrophotometer, centrifuges, and various other items used for our wet lab experimentation.

 

DLS, ITC and Fermenter

Dynamic Light scattering (Wyatt DynaPro Titan), Isothermal Titration Calorimetry (Microcal ITC 200) and 14 liters bioreactor fermenter (BioFlo 310) instrumentation available in the Moffitt Structural Biology Core (SBC) facility.