Jinyi Zhu

Research Scientist

Crystallography, Protein Chemistry, Biochemistry

Email: jinyi.zhu@moffitt.org


Research Scientist H. Lee Moffitt Cancer Center, Tampa, FL 2013 - Present

Director, Protein Production Core Department of Molecular Medicinem, University of South Florida 2012 - 2013

Postdoctoral Fellow H. Lee Moffitt Cancer Center, Tampa, FL 2008 - 2012

Research Assistant University of Georgia, Athens, GA, 2004 - 2008


Doctor of Philosophy in Chemistry 08/2003-08/2008
University of Georgia, Athens, GA, USA Department of Molecular Medicinem, University of South Florida 2012 - 2013

Bachelor of Science in Chemistry 09/1998-06/2002
Nanjing University, Nanjing, China


  • Olesen SH, Ingles DJ, Zhu JY, Martin MP, Betzi S, Georg GI, Tash JS, Schonbrunn E. Stability of the Human Hsp-90p50cdc37 Chaperone Complex against Nucleotides and Hsp90 Inhibitors, and the influence of Phosphorolaytion by Casien Kinase 2. Journal Molecules, 2015 25608045
  • Ember SW*, Zhu JY*, Olesen SH, Martin MP, Beckker A, Berndt N, Georg GI, Schönbrunn E. Acetyl-lysine binding site of bromodomain-containing protein 4 (BRD4) interacts with diverse kinase inhibitors. 2014 PubMed ID: 24317075
  • Zhu JY, Fu ZQ, Chen L, Xu H, Chrzas J, Rose JP, Wang BC. Structure of the Archaeoglobus fulgidus orphan ORF AF1382 determined by sulfur-SAD from a moderately diffracting crystal. Acta Crystallogr D Biol Crystallogr., 2012. Pubmed ID: 22948926
  • Zhu JY*, Yang Y*, Han H, Betzi S, Marsilio F, Schonbrunn E. Functional consequence of covalent reaction of phosphoenolpyruvate with UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA). J Biol Chem., 2012. Pubmed ID:22378791
  • Martin MP, Zhu JY, Lawrence HR, Pirredu R, Luo Y, Alam R, Ozcan S, Sebti SM, Lawrence NJ, Schonbrunn E. A novel mechanism by which small molecule inhibitors induce the DFG flip in Aurora A. ACS Chem Biol., 2012. Pubmed ID: 22248356
  • Li R, Martin MP, Liu Y, Wang B, Patel RA, Zhu JY, Sun N, Pireddu R, Lawrence NJ, Li J, Haura EB, Sung SS, Guida WC, Schonbrunn E, Sebti SM. Fragment-based and structure-guided discovery and optimization of Rho kinase inhibitors. J Med Chem., 2012. Pubmed ID: 22272748
  • Lee JC, Francis S, Dutta D, Gupta V, Yang Y, Zhu JY, Tash JS, Schonbrunn E, Georg GI. Synthesis and evaluation of eight- and four-membered iminosugar analogues as inhibitors of testicular ceramide-specific glucosyltransferase, testicular β-glucosidase 2, and other glycosidases. J Org Chem., 2012. Pubmed ID: 22432895
  • Martin MP*, Alam R*, Betzi S, Ingles DJ, Zhu JY, Schonbrunn E. A novel approach to the discovery of small-molecule ligands of CDK2. ChemBioChem., 2012. Pubmed ID: 22893598
  • Lawrence H, Martin MP, Luo Y, Pireddu R, Yang H, Gevariya H, Ozcan S, Zhu JY, Kendig R, Rodriguez M, Elias R, Cheng J, Sebti SM, Schonbrunn E, Lawrence NJ. Development of ortho-Chlorophenyl Substituted Pyrimidines as Exceptionally Potent Aurora A Inhibitors. J Med Chem., 2012. Pubmed ID: 22803810
  • Pireddu R, Forinash K, Sun N, Martin MP, Sung SS, Alexander B, Zhu JY, Guida WC, Schonbrunn E, Sebti SM, Lawrence NJ. Pyridylthiazole-based ureas as inhibitors of Rho associated protein kinases (ROCK1 and 2). Med. Chem. Commun., 2012. Pubmed ID: 23275831
  • Li Y, Bahti P, Shaw N, Song G, Chen S, Zhang X, Zhang M, Cheng C, Yin J, Zhu JY, Zhang H, Che D, Xu H, Abbas A, Wang BC, Liu ZJ. Crystal Structure of a novel non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304 solved by S-SAD using a Cr X-ray source. Proteins, 2008. Pubmed ID: 18361456
  • Zhu JY, Liu L, Lu CS, Meng QJ. Inclusion Compound of beta-Cyclodextrin with p-Azidobenzoic Acid, Chinese Journal of Organic Chemistry, 2003.


Professional Skills

Experience in high-throughput protein purification by ÄKTA FPLC, ÄKTA Prime Plus and ÄKTA purifier 3D systems.
Protein modification: reductive methylation.
Experience in protein crystallization using robotic systems: Cartesian Honeybee, Douglas ORYX6 crystallization robot and eDrop/Hydra system; Tecan Genesis RSP robot and Bruker CRYSTAL FARM 400.
X-ray Crystallography skills, including synchrotron data collection and processing, structure determination and structure analysis.


2008 Travel Grant by the 2008 Annual Meeting of the American Crystallographic Association.
2006 ISGO Young Scientist Travel Award by the International Structural Genomics Organization (ISGO) and the NIH Protein Structure Initiative.

Oral/Poster Presentation

J.-Y. Zhu*, Z.-Q. Fu, L. Chen, H. Xu, J. Chrzas, J. Rose and B.C. Wang, An Example of Successful Sulfur SAS Phasing using Medium-Resolution Data: Crystal Structure of Archaeoglobus fulgidus ORF 1382. 2008 Annual Meeting of the American Crystallographic Association, Knoxville, TN, USA, June 2008. (Oral)
J.-Y. Zhu*, J. Hwang, H. Xu, Z.-J. Liu, J. Rose and B.-C. Wang, Establishing A High Throughput Pipeline for the Production of Non-Pfam Proteins. 2006 International Conference on Structural Genomics, Beijing, China, Octpber, 2006. (Poster)

Society Membership

American Crystallographic Association (From 2007)