Wanda Manieri

Wanda Manieri

Postdoctoral Fellow

Protein Chemistry, Enzymology, Molecular Biology

Email: wanda.manieri@moffitt.org

Research

2009 - Present Moffitt Cencer Center

Education

Ph.D on Biochemistry, 2002, University of Neuchâtel (Switzerland), Laboratoire de Biochimie, Institute de Botanique, Neuchâtel

Professional Qualification on Pharmacy, 1997, University of Urbino, Italy

B.S.A in Pharmacy, 1996, University of Urbino (Italy)

Publications

  • Linger BR., Manieri W., and Price CM «Division of Labor Among Telomere Proteins: Different Functional Roles for Overhang Binding Proteins in Tetrahymena thermophila», 2009, in preparation.
  • Manieri W, Wilson J, Kovall r., and Caperelli CA. « Crystal Structure of Human GAR Synthetase domain» 2009, in preparation.
  • Manieri W. and Caperelli C.A. «Expression, Purification and Investigation of the mechanism of action of Human GAR Synthetase domain», 2009, in preparation.
  • Dai S, Friemann R, Glauser DA, Bourquin F, Manieri W, Schurmann P, Eklund H. «Structural snapshots along the reaction pathway of ferredoxin-thioredoxin reductase», Nature. 2007 Jul 5; 448(7149):92-6.
  • Manieri W., Moore M.E., Soellner M.B., Tzang P., Caperelli C.A. «Human Glycinamide Ribonucleotide Transformylase: Active Site Mutants as Mechanistic Probes», Biochemistry, 2007, 46, 156-163.
  • Balmer Y, Vensel WH, Cai N, Manieri W, Schurmann P, Hurkman WJ, Buchanan BB.«A complete ferredoxin/thioredoxin system regulates fundamental processes in amyloplasts»,Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2988-93. Epub 2006 Feb 15.
  • Walters E.M, Garcia-Serres R., Jameson G.N.L, Glauser D.A., Bourquin F., Manieri W., Schürmann P., Johnson M.K. and Huynh B.H. «Spectroscopic Characterization of Site-Specific [Fe4S4] Cluster Chemistry in Ferredoxin:Thioredoxin Reductase: Implication for the Catalytic Mechanism», J Am Chem Soc. 2005 Jul 6;127(26):9612-24
  • Balmer Y., Vensel W.H., Tanaka C.K., Hurkman W.J., Gelhaye E., Rouhier N., Jacquot J-P., Manieri W., Schurmann P., Droux M., Buchanan B.B. «Thoredoxin links redox to the regulation of fundamental processes of plant mitochondria» Proc Natl Acad Sci USA. 2004 Feb 24; 101(8):2642-7
  • Rouhier N., Gelhaye E., Gualberto J., Jordy M-N., De Fay E., Hirasawa M., Duplessis S., Lemaire S., Frey P., Martin F., Manieri W., Knaff D.B., and Jacquot J-P. «Poplar peroxiredoxin Q. A thioredoxin-linked chloroplast antioxidant functional in pathogen defense» Plant Physiol. 2004 Mar; 13(3):1027-38.
  • Glauser D.A, Bourquin F., Manieri W., Schurmann P. «Charactrization of ferrodin:thioredoxin reductase modified by site-directed mutagenesis». J Biol Chem 2004 Apr 16; 279(16):16662-9.
  • Manieri W., Franchini L., Raeber L., Dai S., Stritt-Etter A-L., Schürmann P. «N-terminal truncation of the variable subunit stabilizes spinach ferredoxin:thioredoxin reductase». FEBS Lett 2003 Aug 14; 549(1-3):167-70
  • Jameson G.N.L, Walters E.M., Manieri W., Schürmann P., Johnson M.K., Huynh Hanh. B.H. «Spectroscopic evidence for site specific chemistry at a unique iron site of the [4Fe-4S] cluster in ferredoxin:thioredoxin reductase». J.AM.CHEM.SOC. 2003, Feb 5; 125(5):1146-7
  • Balmer Y., Koller A., del Val G., Manieri W., Schürmann P., Buchanan BB. «Proteomics gives insight into the regulatory function of chloroplast thioredoxins» Proc Natl Acad Sci USA. 2003 Jan 7; 100(1):370-5.
  • Gaymard E., Franchini L., Manieri W., Stutz E., Schürmann P. «A dicistronic construct for the expression of functional spinach chloroplast ferredoxin:thioredoxin reductase in Escherichia coli». Plant Sci 2000 Sep 8; 158(1-2):107-13
  • Hirasawa M., Schürmann P., Jacquot J-P., Manieri W. Jacquot P. Keryer E., Hartman F.C., Knaff D. «Oxidation-reduction properties of chloroplast thioredoxins, ferredoxin:thioredoxin reductase and thioredoxin f-regulated enzymes». Biochemistry, 1999 Apr 20; 38(16):5200-5
  • Schwendtmayer C., Manieri W., Hirasawa M., Knaff DB and Schürmann P., 1998:«Cloning, expression and characterization of ferredoxin:thioredoxin reductase from Synechocystis sp. PCC6803». In Photosynthesis Mechanism and Effects (Proc. Int. Congr. Photosynth. 11th, Budapest, Hungary), ed. G.Garab, 3:1927-30, Dordrecht, The Netherlands, Kluwer.
  • D.B.Knaff, M. Hirasawa, J-P. Jacquot, Manieri W. and P. Schürmann. The oxidation-reduction properties of the plant chloroplast ferredoxin, thioredoxin, fructose 1,6-bisphosphatase system. Photosynthesis: Mechanism and Effects. G. Garab. Garab G., Dordrecht:Kluwer Academic Publishers. 3 (ISBN 0-7923-5547-4): 1943-1946, 1988. 08-17-2098.5 121.

Resume

Research Experience

2008-2009 Post-Doctoral Fellow: “Telomere and DNA Damage Response”. Team director: Prof. Carolyn M. Price, Department of Cancer & Cell Biology, University of Cincinnati, Ohio, USA.

2003-2008 Post-Doctoral Fellow: “Characterization of human GARS-AIRS-GART Trifunctional enzyme in the de novo purine biosynthesis modified by site-directed mutagenesis”. Team director: Prof. Carol A. Caperelli, Medical Center, College of Pharmacy, University of Cincinnati, OHIO USA.

2002-2003 Post-Doctoral Fellow: “The Regulated Photosynthetic Chloroplast Enzymes System”. Team director: Prof. Peter Schürmann, University of Neuchâtel, Switzerland.

1998-2002 Ph.D student: “The Ferredoxin/Thioredoxin regulated system”. Work director: Prof. Peter Schürmann, Institute of Biochemistry, University of Neuchâtel, Switzerland.

1992-1996 Pharmacy student: Ins (1,4,5)P3 in the development and morphogenesis of lycopersicum esculentum Mill. in vitro. Work director Prof. Augusto Accorsi, Department of Pharmacy, Institut Biochemistry, University of Urbino, Italy.

Research Support/Honors

Post-graduate Research fellowship “Erasmus” of $25,000 issued from the European Community, 1997.

Selected coursework

1997 Rapid Protein Purification

1998 Redox-potential determination by Fluorometry

2009 RAM Advance course work.