We study how nitric oxide (NO) mediates resistance to immune-based therapies. We utilize specific techniques to measure antigen presentation, multidimensional flow cytometry for phenotyping of cells and functional characterization, and multiplexed immunofluorescence to determine the cell compositions and spatial arrangements between cells. These techniques are used to study these phenomena in both human specimens and murine models.

In the figure below we show a multidimensional flow cytometry experiment and our informatics workflow allowing for efficient analysis.